Volume 2, Issue 1 (Winter-Spring 2019)
Mod Med Lab J 2019, 2(1): 96-99 |
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Rashmi Chandraker 
, Vanita C.Rathod , Neeraj K. Chandraker , Siddharth Pundir , Sudhanshu Dixit , Veena Desai
, Vanita C.Rathod , Neeraj K. Chandraker , Siddharth Pundir , Sudhanshu Dixit , Veena Desai
Abstract: (16701 Views)
Background: Clearing is an important step in the preparation of histological sections, that removes alcohol and other dehydrants from tissues prior to infiltration of the embedding material (usually paraffin wax).Different types of clearing agents are chloroform, Xylene, Toluene, Paraffin, Methyl benzoate and methyl salicylate & Citrus fruit oils.The commonly used clearing agent is xylene that is miscible with both alcohol & parrafin wax. Xylene is supposed to be highly toxic and carcinogenic. As previous research studies have shown the effectiveness of different vegetable oils as clearants, this study was designed to evaluate the efficacy of coconut oil.
Materials and Methods: Two equal halves of 25 oral soft tissue specimens were processed simultaneously in xylene and coconut oil as clearing agents. The Xylene‑treated specimens (XY‑S) and Coconut oil–treated specimens (CO‑S) were checked for gross and histological features and comparison was done between the two groups.
Results: Significant shrinkage was noted in XY‑S compared to that in CO‑S. No difference was found in either of the sections when checked for cellular details and staining quality. Morphometrically, there was significant reduction in the mean cell area in XY‑S compared to that in CO‑S.
Conclusion: Coconut oil may be substituted for the highly hazardous xylene as a clearing agent without compromising the quality of histological details.
Materials and Methods: Two equal halves of 25 oral soft tissue specimens were processed simultaneously in xylene and coconut oil as clearing agents. The Xylene‑treated specimens (XY‑S) and Coconut oil–treated specimens (CO‑S) were checked for gross and histological features and comparison was done between the two groups.
Results: Significant shrinkage was noted in XY‑S compared to that in CO‑S. No difference was found in either of the sections when checked for cellular details and staining quality. Morphometrically, there was significant reduction in the mean cell area in XY‑S compared to that in CO‑S.
Conclusion: Coconut oil may be substituted for the highly hazardous xylene as a clearing agent without compromising the quality of histological details.
Type of Study: Brief Original Article |
Subject:
Clinical Microbiology
References
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2. .Jeyanthi PK et al.Tissue processing of oral biopsy specimens-an adjunct to diagnosis.Journal of Craniomaxillary Diseases 2013, 2(1).
3. Sudeendra P, Acharya s, Javali SB & Wajid S. Comparing the efficacy of coconut oil and xylene as a clearing agent in the histopathology laboratory. Journal of Oral and Maxillofacial Pathology 2014, 18(1)
4. Andre GG, Wenger JB, Rebolloso D, Arrington JB, Mehm WJ. Evaluation of clearing and infiltration mixtures (CIMs) as xylene substitutes for tissue processing. J Histotechnol 1994;17:137‑42 [DOI:10.1179/his.1994.17.2.137]
5. Udkong M et al. Bleached Palm Oil As Substitute For Xylene In Histology 2014,8
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